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One of the greatest fringe benefits is the pleasure and positive vibe from getting your assay to work.  After fighting a need to survive and create good reliable data, there’s the hell of finding out your assay doesn’t work.  Follow that by spending a month or two fighting the odds, searching for tubes that don’t exist, transforming strains, and searching for what’s not working, you step back, and yes, your tube changes color, and it’s done!  Your assay works.  That single moment of joy and accomplishment makes the struggle of 2 months worth it.  There’s no question, you’ve accomplished something.  On to the next experiment with a little more confidence.