dcbio.org

Finally.  We tried a few protocols, borrowed one from a functional genomics class using Tri Reagent and Phase Lock Gel Tubes from 5-prime.  Success.  2.5µg/µl of RNA.  Which is great for our needs.

I remember the isolation of a small industrial lab that I worked in long ago.  If there was a product in need, like media, or a chemical, you were out of luck and had to wait for Fisher to ship.  Production could have been delayed.

One of the greatest things about being in an academic environment. If you need to borrow a chemical or something isn’t working, you can knock on a door and compare notes with another researcher.  Add the interactive environment between PI’s, post docs, lab techs, student researchers, and you have a real community that supports a productive research environment.

Thanks to everyone for their help.  We couldn’t have completed today’s research if we didn’t trade a bag of green tea and brow rice for a few 2ml screw cap tubes…